Nervous system wild type embryos were examined using bright field, confocal,
and two-photon microscopies. Antibody staining specific for the central and
peripheral nervous system was performed. DAB (diaminobenzidine) staining was
used for visualization under a brightfield microscope. Flurescent dyes were
used if specimens were observed using confocal or two-photon microscopies.
Embryos were kept intact to obtain an overal image of the nervous sytem from
the lateral and ventral views. They were dissected (fillet preparation) to
reveal detailed structures of the nervous system.